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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha; Santa Teresa. |
Data corrente: |
10/11/2020 |
Data da última atualização: |
06/12/2022 |
Autoria: |
FONSECA, J. R.; RANGEL, P. H. N.; BEDENDO, I. P.; SILVEIRA, P. M. da; GUIMARÃES, E. P.; CORADIN, L. |
Afiliação: |
Jaime Roberto Fonseca; Paulo Hideo N. Rangel; Ivan Paulo Bedendo; Pedro Marques da Silveira; Elcio Perpétuo Guimarães; Lídio Coradin. |
Título: |
Características botânicas e agronômicas de cultivares e raças regionais de arroz (Oryza sativa L.), coletadas no Estado do Maranhão. |
Ano de publicação: |
1982 |
Fonte/Imprenta: |
Goiania: EMBRAPA-CNPAF; Brasília, DF: EMBRAPA-CENARGEN, 1982 |
Páginas: |
42 p. |
Série: |
(EMBRAPA-CNPAF. Boletim de pesquisa, 1). |
Idioma: |
Português |
Conteúdo: |
Dentre os estados que cultivam arroz, destaca-se o Maranhão, hoje ocupando o 2. lugar em valor de produção. De maneira geral, as cultivares utilizadas são as nativas. O CNPAF e CENARGEN, a EMAPA e técnicos da extensão rural, realizou em 1979, uma expedição para coleta de germoplasma de arroz nas áreas tradicionais da cultura, visando a conservar este material para uso imediato ou futuro em programas de melhoramento de arroz. Dentro deste contexto e objetivando uma melhor utilização deste material em programas de melhoramento conduzidos nesta cultura, o CNPAF desenvolveu um trabalho de caracterização e avaliação de todo o material genético coletado na referida expedição. |
Palavras-Chave: |
Agronomic characters; Brasil; Característica; Caracteristica agronomica; Caracteristica botanica; Caracteristicas botanicas; Coleta; Coleta de germoplasma; Conservacao de germoplasma; Cultivar; Maranhao. |
Thesagro: |
Arroz; Botânica; Brazil; Características Agronômicas; Germoplasma; germplasm conservation; Melhoramento Genético Vegetal; Oryza Sativa; plant breeding; Raça; rice; Variedade. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02068nam a2200469 a 4500 001 1022607 005 2022-12-06 008 1982 bl uuuu u0uu1 u #d 100 1 $aFONSECA, J. R. 245 $aCaracterísticas botânicas e agronômicas de cultivares e raças regionais de arroz (Oryza sativa L.), coletadas no Estado do Maranhão. 260 $aGoiania: EMBRAPA-CNPAF; Brasília, DF: EMBRAPA-CENARGEN$c1982 300 $a42 p. 490 $a(EMBRAPA-CNPAF. Boletim de pesquisa, 1). 520 $aDentre os estados que cultivam arroz, destaca-se o Maranhão, hoje ocupando o 2. lugar em valor de produção. De maneira geral, as cultivares utilizadas são as nativas. O CNPAF e CENARGEN, a EMAPA e técnicos da extensão rural, realizou em 1979, uma expedição para coleta de germoplasma de arroz nas áreas tradicionais da cultura, visando a conservar este material para uso imediato ou futuro em programas de melhoramento de arroz. Dentro deste contexto e objetivando uma melhor utilização deste material em programas de melhoramento conduzidos nesta cultura, o CNPAF desenvolveu um trabalho de caracterização e avaliação de todo o material genético coletado na referida expedição. 650 $aArroz 650 $aBotânica 650 $aBrazil 650 $aCaracterísticas Agronômicas 650 $aGermoplasma 650 $agermplasm conservation 650 $aMelhoramento Genético Vegetal 650 $aOryza Sativa 650 $aplant breeding 650 $aRaça 650 $arice 650 $aVariedade 653 $aAgronomic characters 653 $aBrasil 653 $aCaracterística 653 $aCaracteristica agronomica 653 $aCaracteristica botanica 653 $aCaracteristicas botanicas 653 $aColeta 653 $aColeta de germoplasma 653 $aConservacao de germoplasma 653 $aCultivar 653 $aMaranhao 700 1 $aRANGEL, P. H. N. 700 1 $aBEDENDO, I. P. 700 1 $aSILVEIRA, P. M. da 700 1 $aGUIMARÃES, E. P. 700 1 $aCORADIN, L.
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Registro original: |
Santa Teresa (BST) |
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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
09/09/2020 |
Data da última atualização: |
09/09/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
DUARTE, M. F.; PEREIRA-CARVALHO, R. de C.; REIS, L. de N. A. dos; ROJAS, M. J.; GILBERTSON, R.; COSTA, H.; BOITEUX, L. S.; FONSECA, M. E. N. |
Afiliação: |
Macaria Ferreira Duarte, UnB; Rita de Cássia Pereira Carvalho, UnB; Luciane de Nazaré Almeida dos Reis, UnB; Maria J. Rojas, UC-DAVIS; Robert Gilbertson, UC-DAVIS; Helcio Costa, Incaper; Leonardo Silva Boiteux, EMBRAPA; Maria Esther N. Fonseca, EMBRAPA. |
Título: |
Natural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Plant Disease, set. 2020. |
Idioma: |
Inglês |
Conteúdo: |
Severe yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop. MenosSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia ... Mostrar Tudo |
Thesagro: |
Doença de Planta; Erva Daninha; Mosaico; Tomate. |
Thesaurus NAL: |
Begomovirus. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/4073/1/natural-infection-tomatoes-costa.pdf
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Marc: |
LEADER 04125naa a2200265 a 4500 001 1022277 005 2020-09-09 008 2020 bl uuuu u00u1 u #d 100 1 $aDUARTE, M. F. 245 $aNatural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States.$h[electronic resource] 260 $c2020 520 $aSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop. 650 $aBegomovirus 650 $aDoença de Planta 650 $aErva Daninha 650 $aMosaico 650 $aTomate 700 1 $aPEREIRA-CARVALHO, R. de C. 700 1 $aREIS, L. de N. A. dos 700 1 $aROJAS, M. J. 700 1 $aGILBERTSON, R. 700 1 $aCOSTA, H. 700 1 $aBOITEUX, L. S. 700 1 $aFONSECA, M. E. N. 773 $tPlant Disease, set. 2020.
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